Transduction of peptides, proteins and nucleotides into live cells by cell penetrating peptides
نویسندگان
چکیده
Cell membranes are permeable only for small hydrophobic compounds. While in-vitro transfection studies can be performed with viral factors, electroporation, magneto-fection or application of lipid detergents the internalization of cargos for medically relevant purposes requires more gentle methods such as use of nano-particles or cell-penetrating peptides (CPPs). Especially the formation and internalization of non-covalent complexes between CPPs and cargos meets the requirements for application in diagnosis and therapy.
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متن کاملTransduction of peptides and proteins into live cells by cell penetrating peptides.
Internalization of peptides and proteins into live cells is an essential prerequisite for studies on intracellular signal pathways, for treatment of certain microbial diseases and for signal transduction therapy, especially for cancer treatment. Cell penetrating peptides (CPPs) facilitate the transport of cargo-proteins through the cell membrane into live cells. CPPs which allow formation of no...
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Modulating signaling pathways for research and therapy requires either suppression or expression of selected genes or internalization of proteins such as enzymes, antibodies, nucleotide binding proteins or substrates including nucleoside phosphates and enzyme inhibitors. Peptides, proteins and nucleotides are transported by fusing or conjugating them to cell penetrating peptides or by formation...
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Modulating signaling pathways for research and therapy requires either suppression or expression of selected genes or internalization of proteins such as enzymes, antibodies, nucleotide binding proteins or substrates including nucleoside phosphates and enzyme inhibitors. Peptides, proteins and nucleotides are transported by fusing or conjugating them to cell penetrating peptides or by formation...
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Cell penetrating peptides (CPP), also known as protein transduction domains (PTD), are small peptides able to carry peptides, proteins, nucleic acid, and nanoparticles, including viral particles, across the cellular membranes into cells, resulting in internalization of the intact cargo. In general, CPPs can be broadly classified into tissue-specific and non-tissue specific peptides, with the la...
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